MiRAGE
In a joint effort of academia and industry, we establish an automated countercurrent flow reactor for enzymatic glycan synthesis by microgel-immobilized glycosyltransferases. Partners form Laboratory for Biomaterials of the RWTH Aachen University, the Institute for Technical and Macromolecular Chemistry of the Leibniz Institute for Interactive Materials, the Institute of Functional Interfaces of the Karlsruhe Institute of Technology and the glyXera GmbH work on different task in this collaboration.
Microgel
Permeable hydrogel network for enzyme immobilization with homogenous flow properties
countercurrent flow reactor
Device suitable for enzymatic reactions with countercurrent substrate/product- and auxiliary buffer-flow
for automated
A programmable, scalable process with at-line glycan analytics
glycan synthesis
Oligosaccharides with different epitopes
with immobilized enzymes
Glycosyltransferases that are covalently bound to hydrogel and can be reused for several cycles
During the funding period, interdisciplinary expertise from the different partners is applied to reach sophisticated milestones.
During the funding period, interdisciplinary expertise from the different partners is applied to reach sophisticated milestones.
➤Immobilization of glycosyltransferases in functional and biocompatible microgels
➤Design and construction of a membrane based counterflow reactor system coupled with product purification and concentration.
➤In silico simulation of immobilized enzyme cascades for optimization of bio-processing.
The project started in February 2022 and is funded with over 2.1 million euro by the National Bioeconomy Strategy Initiative of the German Federal Ministry of Education and Research as part of the program "Future Technologies for the Industrial Bioeconomy: Focus on Biohybrid Technologies.